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	We will be exhibiting at the ISF World Seed Congress 2026 in Lisbon, May 18-20. Join the flagship annual gathering of the global seed sector.ISF World Seed Congress 2026 · Lisbon 18-20 May · Join the flagship annual gathering of the global seed sector. Our Current Booth Selection is 2.20.

We will be exhibiting at the ISF World Seed Congress 2026 in Lisbon, May 18-20.

Join the flagship annual gathering of the global seed sector.ISF World Seed Congress 2026 · Lisbon 18-20 May · Join the flagship annual gathering of the global seed sector.

Our Current Booth Selection is 2.20.

SOLUTION

Multiplex rapid testing method for pathogens in vegetable seeds.

Features of the DNA chip testing method.

Existing seed testing methods require substantial time, money, and work because different methods must be conducted for different pathogens. Our new method with simultaneous DNA detection will drastically reduce time and cost to verify seed health.

TESTING PROCEDURES

Vegetable seed testing procedures

The new method tests nucleic acids extracted from seed samples with multiplex PCR and hybridization on a DNA chip, achieving multiple pathogens detection.

TECHNOLOGY

Key features of the new test method

Simple operation
Simultaneous DNA testing drastically simplifies the process of preparing reagents.
High precision
The two-step approach using PCR and hybridization can provide more reliable results with precise detection of target DNA.

	Multiplex PCR Selective and sensitive amplification and detection of multiple target nucleic acids achieved by techniques for: ◆1 Designing primers ◆2 Determining primer amounts and mixing ratios ◆3 Setting PCR conditions ◆4 Designing DNA probes
	DNA chips 30 years of technology to manufacture DNA chips features: ◆1 Simultaneous detection of 64 pathogens ◆2 More accurate detection with a higher signal-noise ratio ◆3 High cost performance with mass production technology
	Automatic testing device High throughput ・Up to 96 DNA chips can be measured in a single testing operation. ・It takes just 60-90 minutes from setting up the device to obtaining test results. Reliability ・Equipped with a QR code scanning function for sample registration, enabling accurate matching with test results. Can be used under standard lab conditions ・Compact tabletop size (W900 x D650 x H700 mm) ・Power supply: AC 100-240 V

Multiplex PCR

Selective and sensitive amplification and detection of multiple target nucleic acids achieved by techniques for:
◆1 Designing primers
◆2 Determining primer amounts and mixing ratios
◆3 Setting PCR conditions
◆4 Designing DNA probes

DNA chips

30 years of technology to manufacture DNA chips features:
◆1 Simultaneous detection of 64 pathogens
◆2 More accurate detection with a higher signal-noise ratio
◆3 High cost performance with mass production technology

Automatic testing device

High throughput
・Up to 96 DNA chips can be measured in a single testing operation.
・It takes just 60-90 minutes from setting up the device to obtaining test results.

Reliability
・Equipped with a QR code scanning function for sample registration, enabling accurate matching with test results.

Can be used under standard lab conditions
・Compact tabletop size (W900 x D650 x H700 mm)
・Power supply: AC 100-240 V

PRODUCTS

Testing kits for major vegetable families

We plan to launch eight types of seed testing kits,including DNA chips and multiplex PCR reagents,during the period from 2025 to 2027.

Quality control inspection kit

Export inspection kit

Joint development with Thai DOA Khon Kaen and TALENOME

	35 pathogen in Tomato detection Fluorescent hybridization by using GENOGATE CHIP A Genogate chip made from a special type of plastic that has excellent DNA-binding properties. The 3 mm Genogate chip is located at the center of a plastic slide and has a large number of DNA fragments immobilized on its surface. It is used for detecting target DNA. The sample DNA that perfectly complements the DNA immobilized on the chip (universal probe) will bind to it through the principle of hybridization, which is the pairing of complementary DNA sequences—similar to the natural base pairing in DNA strands: A pairs with T, and G pairs with C. When the target DNA binds to the DNA on the chip, which includes a primer labeled with a fluorescent dye, and hybridization is complete, the chip must be washed to remove any unbound DNA. This leaves only the target DNA bound to the chip. The chip is then scanned with a scanner to detect the resulting fluorescence signal. Pathogen List Fungi 1. Cercospora fuligena 2. Phytophthora capcisi 3. Phytophthora infestans 4. Fusarium oxysporum f.sp.lycopersici 5. Fusarium oxysporum f.sp.lycopersici RACE3 Bacteria 6. Clavibacter michiganensis subsp. michiganensis 7. Pseudomonas corrugata 8. Pseudomonas syringae pv. Tomato 9. Ralstonia solanacearum 10. Xanthomonas vesicatoria 11. Xanthomonas euvesicatoria 12. Xanthomonas perforans 13. Xanthomonas hortorum pv. Gardneri Viroid 14. Potato spindle tuber viroid (PSTVd) 15. Citrus exocortis viroid (CEVd) 16. Columnea latent viroid (CLVd) 17. Pepper chat fruit viroid (PCFVd) 18. Hop stunt viroid (HSVd) 19. Chrysanthemum stunt viroid (CSVd) 20. Tomato chlorotic dwarf viroid (TCDVd) 21. Tomato apical stunt viroid (TASVd) 22. Tomato bunchy top viroid (TBTVd) 23. Tomato planta macho viroid (TPMVd) 24. Cucumber pale fruit viroid (CPFVd) Virus 25. Tomato brown rugose fruit virus (ToBRFV ) 26. Pelargonium zonate spot virus (PZSV) 27. Pepino mosaic virus (PMV) 28. Tobacco ringspot virus (TRV) 29. Tobacco streak virus (TSV) 30. Tobamo: Tobacco mild green mosaic virus (TMGM), Tobacco mosaic virus (TMV),Tomato mosaic virus (ToMV) 31. Tomato black ring virus (TBRV) 32. Tomato bushy stunt virus (TBSV) 33. Tomato ringspot virus (ToRSV) 34. Tomato spotted wilt virus (TSWV) 35. Tomato yellow leaf curl virus (TYLCV)

35 pathogen in Tomato detection Fluorescent hybridization by using GENOGATE CHIP

A Genogate chip made from a special type of plastic that has excellent DNA-binding properties.
The 3 mm Genogate chip is located at the center of a plastic slide and has a large number of DNA fragments immobilized on its surface. It is used for detecting target DNA.
The sample DNA that perfectly complements the DNA immobilized on the chip (universal probe) will bind to it through the principle of hybridization, which is the pairing of complementary DNA sequences—similar to the natural base pairing in DNA strands: A pairs with T, and G pairs with C. When the target DNA binds to the DNA on the chip, which includes a primer labeled with a fluorescent dye, and hybridization is complete, the chip must be washed to remove any unbound DNA.
This leaves only the target DNA bound to the chip. The chip is then scanned with a scanner to detect the resulting fluorescence signal.

Pathogen List

Fungi
1. Cercospora fuligena
2. Phytophthora capcisi
3. Phytophthora infestans
4. Fusarium oxysporum f.sp.lycopersici
5. Fusarium oxysporum f.sp.lycopersici RACE3

Bacteria
6. Clavibacter michiganensis subsp. michiganensis
7. Pseudomonas corrugata
8. Pseudomonas syringae pv. Tomato
9. Ralstonia solanacearum
10. Xanthomonas vesicatoria
11. Xanthomonas euvesicatoria
12. Xanthomonas perforans
13. Xanthomonas hortorum pv. Gardneri

Viroid
14. Potato spindle tuber viroid (PSTVd)
15. Citrus exocortis viroid (CEVd)
16. Columnea latent viroid (CLVd)
17. Pepper chat fruit viroid (PCFVd)
18. Hop stunt viroid (HSVd)
19. Chrysanthemum stunt viroid (CSVd)
20. Tomato chlorotic dwarf viroid (TCDVd)
21. Tomato apical stunt viroid (TASVd)
22. Tomato bunchy top viroid (TBTVd)
23. Tomato planta macho viroid (TPMVd)
24. Cucumber pale fruit viroid (CPFVd)

Virus
25. Tomato brown rugose fruit virus (ToBRFV )
26. Pelargonium zonate spot virus (PZSV)
27. Pepino mosaic virus (PMV)
28. Tobacco ringspot virus (TRV)
29. Tobacco streak virus (TSV)
30. Tobamo: Tobacco mild green mosaic virus (TMGM), Tobacco mosaic virus (TMV),Tomato mosaic virus (ToMV)
31. Tomato black ring virus (TBRV)
32. Tomato bushy stunt virus (TBSV)
33. Tomato ringspot virus (ToRSV)
34. Tomato spotted wilt virus (TSWV)
35. Tomato yellow leaf curl virus (TYLCV)

Automatic DNA chip testing device

・Designed for GENOGATE DNA chip test kits
・Powerful performance
　- 96 samples / run, 1 hour / run
・Easy to operate
　- single-person use
・Low maintenance
・Compact desktop footprint
　(90 cm wide)

Japan Consortium

Development of a Simultaneous Testing Method for Seed Hazard Pathogens Research Consortium

Funded by Japan's Ministry of Agriculture, Forestry and Fisheries (MAFF) via BRAIN, this project develops a rapid, multiplex DNA chip testing method to detect diverse pathogens in vegetable seeds for enhanced biosecurity and global trade. The initiative, collaborating with private industry, aims for international standardization of these diagnostic protocols to ensure disease-free seed distribution.
More information can be found in the BRAIN project documentation.

Click here for details

Development of a Simultaneous Testing Method for Seed Hazard Pathogens Research Consortium

Company Information	Toyo Seikan Group Holdings, Ltd. Toyo Seikan Group was founded in 1917. We manufacture a variety of packaging containers using materials such as metal, plastic, paper, and glass, and are currently one of the world's leading comprehensive container manufacturers. In addition to 45 domestic companies (including holdings), we have also established 49 group companies overseas, and are expanding our business globally.
Capital	11,094,600,000 yen
Representative	President and CEO Takuji Nakamura The entire group will work together to promote the creation of new value, aiming to become a "Daily Living Platform" that provides safety, security, and prosperity to all people around the world.
Founding	June 25, 1917 (Taisho 6) Toyo Seikan Co., Ltd. (now Toyo Seikan Group Holdings, Ltd.) is founded, with its head office and Osaka factory established in Osaka City (June 25th)
Main Office	〒141-8627 2-18-1 Higashigotanda, Shinagawa-ku, Tokyo Osaki Forest Building
Phone	Phone 03-4514-2000 FAX 03-3280-8111
Number of Employees	495 (as of March 31, 2025) *Consolidated number of employees: 18,830 (as of March 31, 2025)
Business Content	Management of group companies, etc. Beyond the boundaries of packaging,Towards new value creation As a leading container and packaging company, we support people in all sorts of situations. As well as continuing to provide our products, "Food and Health," "Comfortable Living," "Environment, Resources, and Energy" We will continue to work to create new value in these three areas.

CONTACT

Contact

12-2 Ogimachi,Kawasaki-ku,Kawasaki-shi,Kanagawa 210-0867,Japan.
GENETIC TESTING GROUP BUSINESS INNOVATION OFFICE
Tel: +81-44-329-1715
Mail:genogate_inquiry@tskg-hd.com

PROMOTING THE GLOBAL DISTRIBUTION OF HEALTHY VEGETABLE SEEDS.

TESTING KITS FOR MAJOR VEGETABLE FAMILIES.

We will be exhibiting at the ISF World Seed Congress 2026 in Lisbon, May 18-20.

Multiplex rapid testing method for pathogens in vegetable seeds.

Features of the DNA chip testing method.

Vegetable seed testing procedures

Key features of the new test method

Multiplex PCR

DNA chips

Automatic testing device

Testing kits for major vegetable families

Quality control inspection kit

Export inspection kit

35 pathogen in Tomato detection Fluorescent hybridization by using GENOGATE CHIP

Automatic DNA chip testing device

Development of a Simultaneous Testing Method for Seed Hazard Pathogens Research Consortium

Beyond the boundaries of packaging,Towards new value creation

Contact